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Neuropathy was evaluated by sciatic motor (MNCV) and hind-limb digital sensory (SNCV) nerve conduction velocities, thermal response latency (Hargreaves method), mechanical withdrawal threshold (Randall-Selitto test), tactile response threshold (flexible von Frey filament test) and intraepidermal nerve fiber density (fluorescent immunohistochemistry with confocal microscopy)